Book chapter: Wreden CC, Heckscher ES. The Study of Sensorimotor Circuit Assembly in Drosophila melanogaster Embryos and Larvae. Cold Spring Harbor Protocols. 2024. doi: 10.1101/pdb.top108395. Editors: Zhang B, Heckscher ES, Keene AC, Waddell S.

Book chapter: Greaney MR, Wreden CC, Heckscher ES. Studying Drosophila Larval Behavior in Agarose Channels. Cold Spring Harbor Protocols. 2024. doi: 10.1101/pdb.prot108420. PubMed PMID: 39284632. Editors: Zhang B, Heckscher ES, Keene AC, Waddell S.

Book chapter: Vasudevan D, Wreden CC, Heckscher ES. Imaging Neural Activity in Intact, Semirestrained Drosophila Larvae. Cold Spring Harbor Protocols. 2024. doi: 10.1101/pdb.prot108421. PubMed PMID: 39284629. Editors: Zhang B, Heckscher ES, Keene AC, Waddell S.

Book chapter: Marshall ZD, Wreden CC, Heckscher ES. Single-Neuron Labeling in Drosophila Using Multicolor FLP-Out. Cold Spring Harbor Protocols. 2024. doi: 10.1101/pdb.prot108422. Editors: Zhang B, Heckscher ES, Keene AC, Waddell S.

Book chapter: Henderson JE, Wreden CC, Heckscher ES. Fluorescent In Situ Hybridization Chain Reaction for RNA in the Drosophila Embryonic and Larval Central Nervous System. Cold Spring Harbor Protocols. 2024. doi: 10.1101/pdb.prot108423. PubMed PMID: 39284633. Editors: Zhang B, Heckscher ES, Keene AC, Waddell S.

Vasudevan D, Wang Y, Kuskowski E, Corcoran S, Wreden CC, Heckscher ES. Temporal cohort number, neural composition, and connectivity vary along the Drosophila larval body axis. bioRxiv. 2024 Nov 10. doi: 10.1101/2024.11.09.622783.

Diao F, Vasudevan D, Heckscher ES, White BH. Hox gene-specific cellular targeting using split intein Trojan exons. PNAS. 2024 Apr 23;121(17), e2317083121. doi: 10.1073/pnas.2317083121. PubMed PMID: 38602904

Greaney MR, Wreden CC, Heckscher ES. Distinctive features of the central synaptic organization of Drosophila larval proprioceptors. Frontiers in Neural Circuits. 2023 Jul 26;17. doi: 10.3389/fncir.2023.1223334. PubMed PMID: 37564629

Wang Y*, Wreden CC*, Levy M, Meng JL, Marshall ZD, MacLean JN, Heckscher ES. Sequential addition of neuronal stem cell temporal cohorts generates a feed-forward circuit in the Drosophila larval nerve cord. Elife. 2022 Jun 20;11. doi: 10.7554/eLife.79276. PubMed PMID: 35723253 (*equal contributions)

Marshall ZD, Heckscher ES. The role of Even-skipped in Drosophila larval somatosensory circuit assembly. eNeuro. 2022 Feb 2;9(1):ENEURO.0403-21.2021. doi: 10.1523/ENEURO.0403-21.2021. PubMed PMID: 35031555

Book chapter: Meng JL, Heckscher ES. Development of motor circuits: From neuronal stem cells and neuronal diversity to motor circuit assembly. Curr Top Dev Biol. 2021;142:409-442. doi: 10.1016/bs.ctdb.2020.11.010. Epub 2020 Dec 19. PubMed PMID: 33706923. Editor: Bashaw GJ.

Chi W, Liu W, Fu W, Xia S, Heckscher ES, Zhuang X. RNA-binding protein syncrip regulates starvation-induced hyperactivity in adult Drosophila. PLoS Genet. 2021 Feb;17(2):e1009396. doi: 10.1371/journal.pgen.1009396. PubMed PMID: 33617535

Meng JL, Wang Y, Carrillo RA, Heckscher ES. Temporal transcription factors determine circuit membership by permanently altering motor neuron-to-muscle synaptic partnerships. Elife. 2020 May 11;9. doi: 10.7554/eLife.56898. PubMed PMID: 32391795

Meng JL, Marshall ZD, Lobb-Rabe M, Heckscher ES. How prolonged expression of Hunchback, a temporal transcription factor, re-wires locomotor circuits. Elife. 2019 Sep 10;8. doi: 10.7554/eLife.46089. PubMed PMID: 31502540

He L, Gulyanon S, Mihovilovic Skanata M, Karagyozov D, Heckscher ES, Krieg M, Tsechpenakis G, Gershow M, Tracey WD Jr. Direction Selectivity in Drosophila Proprioceptors Requires the Mechanosensory Channel Tmc. Curr Biol. 2019 Mar 18;29(6):945-956.e3. doi: 10.1016/j.cub.2019.02.025. PubMed PMID: 30853433

Wreden CC, Meng JL, Feng W, Chi W, Marshall ZD, Heckscher ES. Temporal Cohorts of Lineage-Related Neurons Perform Analogous Functions in Distinct Sensorimotor Circuits. Curr Biol. 2017 May 22;27(10):1521-1528.e4. doi: 10.1016/j.cub.2017.04.024. PubMed PMID: 28502656

Hirono K, Kohwi M, Clark MQ, Heckscher ES, Doe CQ. The Hunchback temporal transcription factor establishes, but is not required to maintain, early-born neuronal identity. Neural Dev. 2017 Jan 31;12(1):1. doi: 10.1186/s13064-017-0078-1. PubMed PMID: 28137283

Sun X, Heckscher ES. Using Linear Agarose Channels to Study Drosophila Larval Crawling Behavior. J Vis Exp. 2016 Nov 26;(117). doi: 10.3791/54892. PubMed PMID: 27929468

Ellie’s Previous Work (select publications):

Clark MQ, McCumsey SJ, Lopez-Darwin S, Heckscher ES, Doe CQ. Functional Genetic Screen to Identify Interneurons Governing Behaviorally Distinct Aspects of Drosophila Larval Motor Programs. G3 (Bethesda). 2016 Jul 7;6(7):2023-31. doi: 10.1534/g3.116.028472. PubMed PMID: 27172197

Heckscher ES, Zarin AA, Faumont S, Clark MQ, Manning L, Fushiki A, Schneider-Mizell CM, Fetter RD, Truman JW, Zwart MF, Landgraf M, Cardona A, Lockery SR, Doe CQ. Even-Skipped(+) Interneurons Are Core Components of a Sensorimotor Circuit that Maintains Left-Right Symmetric Muscle Contraction Amplitude. Neuron. 2015 Oct 21;88(2):314-29. doi: 10.1016/j.neuron.2015.09.009. PubMed PMID: 26439528

Heckscher ES*, Long F*, Layden MJ, Chuang CH, Manning L, Richart J, Pearson JC, Crews ST, Peng H, Myers E, Doe CQ. Atlas-builder software and the eNeuro atlas: resources for developmental biology and neuroscience. Development. 2014 Jun;141(12):2524-32. doi: 10.1242/dev.108720. PubMed PMID: 24917506 (*equal contributions)

Manning L, Heckscher ES, Purice MD, Roberts J, Bennett AL, Kroll JR, Pollard JL, Strader ME, Lupton JR, Dyukareva AV, Doan PN, Bauer DM, Wilbur AN, Tanner S, Kelly JJ, Lai SL, Tran KD, Kohwi M, Laverty TR, Pearson JC, Crews ST, Rubin GM, Doe CQ. A resource for manipulating gene expression and analyzing cis-regulatory modules in the Drosophila CNS. Cell Rep. 2012 Oct 25;2(4):1002-13. doi: 10.1016/j.celrep.2012.09.009. PubMed PMID: 23063363

Heckscher ES, Lockery SR, Doe CQ. Characterization of Drosophila larval crawling at the level of organism, segment, and somatic body wall musculature. J Neurosci. 2012 Sep 5;32(36):12460-71. doi: 10.1523/JNEUROSCI.0222-12.2012. PubMed PMID: 22956837

Faumont S, Rondeau G, Thiele TR, Lawton KJ, McCormick KE, Sottile M, Griesbeck O, Heckscher ES, Roberts WM, Doe CQ, Lockery SR. An image-free opto-mechanical system for creating virtual environments and imaging neuronal activity in freely moving Caenorhabditis elegans. PLoS One. 2011;6(9):e24666. doi: 10.1371/journal.pone.0024666. PubMed PMID: 21969859